Coding

Part:BBa_K1420001:Design

Designed by: Stephen C. Heinsch   Group: iGEM14_Minnesota   (2014-09-26)


merA, mercuric reductase from Serratia marcescens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1201
    Illegal NgoMIV site found at 1249
    Illegal NgoMIV site found at 1311
    Illegal NgoMIV site found at 1522
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutagenesis of adenine to guanine at position 1026 to remove EcoRI site.


Source

pDU1358, donated by Anne O. Summers.

References